Journal: Journal of ovarian research
Article Title: Nur77 ameliorates cyclophosphamide-induced ovarian insufficiency in mice by inhibiting oxidative damage and cell senescence.
doi: 10.1186/s13048-024-01532-y
Figure Lengend Snippet: Fig. 6 Nur77 slows down ovarian aging in POF mice. (A) The mRNA expressions of p53, p21, p16, and H2AX in each group after overexpression of Nur77. (B) & (C) The protein expressions of p53, p21, p16, and H2AX in each group after the overexpression of Nur77. (D) Immunofluorescence staining was performed to detect the localization and expression of p16, H2AX in the ovarian tissues of each group after the overexpression of Nur77. Scale bar: 50 μm. Error bars, mean ± SEM. n = 6–8/group. *P < 0.05, **P < 0.01, ***P < 0.001 versus the control group. ns, not significant; #P < 0.05, ##P < 0.01 versus the CTX group
Article Snippet: The PVDF membrane was blocked in TBST containing 5% skim milk for 2 h, Following this, membranes were incubated with rabbit polyclonal anti-Nur77 (1:1,000 dilution; Abcam), rabbit polyclonal anti-p16 (1:1,000 dilution; Abcam), rabbit polyclonal anti-p21 antibodies (1:2,000 dilution; Proteintech), rabbit polyclonal anti-Parkin antibodies (1:1,000 dilution; Abcam) and mouse monoclonal anti-PINK1 antibodies (1:500 dilution; Abcam) at 4 °C.
Techniques: Over Expression, Immunofluorescence, Staining, Expressing, Control